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Infinite Allergy Labs

Specialists in Food and Environmental Allergy Testing

Specialists in Food and Environmental Allergy TestingSpecialists in Food and Environmental Allergy TestingSpecialists in Food and Environmental Allergy Testing

IgE Serum Testing for Allergies vs Skin Prick

As quoted from Medscape:


“Specific IgE testing can be done through skin testing or blood testing. Testing should be based on the clinical scenario.”


In vitro tests assess antigen-specific IgE by testing the patient’s serum. Advantages to this method include the use of a single venipuncture that is not affected by medications. In vitro testing can be performed on patients with affected skin, such as dermatographism or atopic dermatitis. It is also a safer option if the patient is at risk for anaphylaxis. However, these tests are expensive compared with skin testing.


In this method, immunoassays measure interactions between antigens and antigen-specific antibodies. Immunoassays are often referred to as radioallergosorbent (RAST) testing, but that term is outdated because radiation is rarely used today. Current methods include enzyme-linked immunosorbent assay (ELISA), which uses antibodies linked to enzymes, as well as fluorescent enzyme immunoassays (FEIA) and chemiluminescent immunoassays, which use fluorescent generation with an enzyme.”


This method described on Medscape, the enzyme-linked immunosorbent assay or ELISA is the exact methodology performed by Infinite Labs. This is well established and routine. 


As stated by Dr. Portnoy, MD in the publication Appropriate Allergy Testing and Interpretation:

“For the primary care provider, in vitro tests have the advantage that they are readily available, they require a single venipuncture, the office staff do not need to be trained to perform them, there is no risk to the patient since directed exposure to the allergen is avoided, and the tests are not affected if the patient has taken an antihistamine.”

References

 

  1. Mullin G, et.al. Testing for Food Reactions: The Good, the Bad, and the Ugly. Nutr in Clin Pract. 2010; 25(2):192-198.
  2. Atkinson W, et. al. Food Elimination Based on IgG Antibodies in Irritable Bowel Syndrome: A Randomized Controlled Trial. Gut. 2004;53:1459-1464.
  3. Drisko J, et. al. Treating Irritable Bowel Syndrome with a Food Elimination Diet Followed by Food Challenge and Probiotics. J Amer Col Nutr.2006;25(6):514-522.
  4. Karakula-Juchnowicz H, et.al. The food-specific serum IgG reactivity in major depressive disorder patients, irritable bowel syndrome patients and healthy controls. Nutrients. 2018 Apr;10(5):pii:E548.
  5. Mitchell N, et. al. Randomized Controlled Trial of Food Elimination Diet Based on IgG Antibodies for the Prevention of Migraine-Like Headaches. Nutr J. 2011 May;10:85.
  6. Alpay K, et. al. Diet Restriction in Migraine, Based on IgG Against Foods: A Clinical Double-Blind, Randomized, Cross-Over Trial. Cephalgia. 2010 Jul;30(7):829-37.
  7. Aydinlar E, et.al. IgG-Based Elimination Diet in Migraine Plus Irritable Bowel Syndrome. Headache. 2013;53:514-525.
  8. Shakib F, et. al. Study of IgG Sub-Class Antibodies in Patients with Milk Intolerance. Clin Allergy. 1986;16(5):451-458.
  9. Panush RS. Food Induced ("Allergic") Arthritis: Clinical and Serologic Studies. J Rheumatol. 1990 Mar;17(3):291-4.
  10. Coucke F. Food intolerance in patients with manifest autoimmunity. Observational study. Autoimmune Rev. 2018 Nov;17(11):1078-1080.
  11. Bentz S, et. al. Clinical relevance of IgG antibodies against food antigens in Crohn's disease: a double-blind cross-over diet intervention study. Digestion. 2010;81(4):252-64.
  12. Wilders-Truschnig M, et. al. IgG antibodies against food antigens are correlated with inflammation and intima media thickness in obese juveniles. Exp Clin Endocrinol Diabetes. 2008 Apr;116(4):241-5.
  13. Purohit V, Bode JC, Bode C, et al. Alcohol, intestinal bacterial growth, intestinal permeability to endotoxin, and medical consequences: summary of a symposium. Alcohol. 2008;42(5):349-361.
  14. Vanuytsel T, van Wanrooy S, Vanheel H, et al. Psychological stress and corticotropin-releasing hormone increase intestinal permeability in humans by a mast cell-dependent mechanism. Gut. 2014;63(8):1293-1299.
  15. Soderholm JD, Perdue MH. Stress and gastrointestinal tract. II. Stress and intestinal barrier function. Am J Physiology Gastro Liver Physiology. 2001;280(1):G7-g13.
  16. Bjarnason I, Takeuchi K. Intestinal permeability in the pathogenesis of NSAID-induced enteropathy. J Gastro. 2009;44 Suppl 19:23-29.
  17. Bibbo S, Ianiro G, Giorgio V, et al. The role of diet on gut microbiota composition. Eur Rev Med Pharm Sci. 2016;20(22):4742-4749.
  18. Jae Hoon Oh, Moon Kyoo Park, Seong Wook Kim, Je-Kyun Park, A fully automated analyzer for multiple detection of allergen-specific immunoglobulin E, Analytical Methods, 10.1039/C5AY01634G, 7, 20, (8889-8895), (2015). Crossref
  19. Pediatric Allergy and ImmunologyVolume 19, Issue 4 Paediatric allergy diagnosis in primary care is improved by in vitro allergen‐specific IgE testing 
  20. Nutritional disorders resulting from food allergy in children Rosan Meyer Pediatric Allergy and Immunology Volume 29, Issue 7 25 July 2018
  21. Intestinal permeability in children with food allergy on specific elimination diets Kirsi M. Järvinen George N. KonstantinouMariecel Pilapil Marie‐Claire ArrietaSally Noone Hugh A. SampsonJon Meddings Anna Nowak‐Węgrzyn Pediatric Allergy and ImmunologyVolume 24, Issue 6: 02 August 2013
  22. The diagnosis of IgE‐mediated food allergy in childhood G. Du Toit A. Santos G. Roberts A. T. FoxP. Smith G. Lack Pediatric Allergy and ImmunologyVolume 20, Issue 4 26 May 2009
  23. Mast cells and complement system: Ancient interactions between components of innate immunity Daniel Elieh Ali Komi  Farzaneh Shafaghat Petri T. Kovanen  Seppo Meri First published: 23 May 2020 https://doi.org/10.1111/all.14413
  24.  CDC. Allergies. Centers for Disease Control and Prevention. Available at http://www.cdc.gov/healthcommunication/ToolsTemplates/EntertainmentEd/Tips/Allergies.html. February 2, 2011; Accessed: February 29, 2016.
  25.  Adinoff AD, Rosloniec DM, McCall LL, Nelson HS. Immediate skin test reactivity to Food and Drug Administration-approved standardized extracts. J Allergy Clin Immunol. 1990 Nov. 86(5):766-74. [Medline].
  26. Sampson HA. Update on food allergy. J Allergy Clin Immunol. 2004 May. 113(5):805-19; quiz 820. [Medline].
  27.  Sampson HA, Ho DG. Relationship between food-specific IgE concentrations and the risk of positive food challenges in children and adolescents. J Allergy Clin Immunol. 1997 Oct. 100(4):444-51. [Medline].
  28. Nelson HS, Oppenheimer J, Buchmeier A, Kordash TR, Freshwater LL. An assessment of the role of intradermal skin testing in the diagnosis of clinically relevant allergy to timothy grass. J Allergy Clin Immunol. 1996 Jun. 97(6):1193-201. [Medline].
  29. Wood RA, Phipatanakul W, Hamilton RG, Eggleston PA. A comparison of skin prick tests, intradermal skin tests, and RASTs in the diagnosis of cat allergy. J Allergy Clin Immunol. 1999 May. 103(5 Pt 1):773-9. [Medline].
  30. Sampson HA. Utility of food-specific IgE concentrations in predicting symptomatic food allergy. J Allergy Clin Immunol. 2001 May. 107(5):891-6. [Medline].
  31. Nolte H, DuBuske LM. Performance characteristics of a new automated enzyme immunoassay for the measurement of allergen-specific IgE. Summary of the probability outcomes comparing results of allergen skin testing to results obtained with the HYTEC system and CAP system. Ann Allergy Asthma Immunol. 1997 Jul. 79(1):27-34. [Medline].
  32. Liacouras CA, Furuta GT, Hirano I, Atkins D, Attwood SE, Bonis PA. Eosinophilic esophagitis: updated consensus recommendations for children and adults. J Allergy Clin Immunol. 2011 Jul. 128(1):3-20.e6; quiz 21-2. [Medline].
  33. Friedmann PS, Ardern-Jones M. Patch testing in drug allergy. Curr Opin Allergy Clin Immunol. 2010 Aug. 10(4):291-6. [Medline].
  34. Beltrani VS, Beltrani VP. Contact dermatitis. Ann Allergy Asthma Immunol. 1997 Feb. 78(2):160-73; quiz 174-6. [Medline].
  35. Simons FE. Anaphylaxis. J Allergy Clin Immunol. 2010 Feb. 125(2 Suppl 2):S161-81. [Medline].
  36. Lieberman P, Nicklas RA, Oppenheimer J, Kemp SF, Lang DM, Bernstein DI. The diagnosis and management of anaphylaxis practice parameter: 2010 update. J Allergy Clin Immunol. 2010 Sep. 126(3):477-80.e1-42. [Medline].
  37. Sanz ML, Gamboa PM, Antepara I, Uasuf C, Vila L, Garcia-Aviles C. Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to betalactam antibiotics. Clin Exp Allergy. 2002 Feb. 32(2):277-86. [Medline].
  38. Homburger HA, Mauer K, Sachs MI, O'Connell EJ, Jacob GL, Caron J. Serum IgG4 concentrations and allergen-specific IgG4 antibodies compared in adults and children with asthma and nonallergic subjects. J Allergy Clin Immunol. 1986 Mar. 77(3):427-34. [Medline].
  39. Beyer K, Teuber SS. Food allergy diagnostics: scientific and unproven procedures. Curr Opin Allergy Clin Immunol. 2005 Jun. 5(3):261-6. [Medline].
  40. Bernstein IL, Li JT, Bernstein DI, et al. Allergy diagnostic testing: an updated practice parameter. Ann Allergy Asthma Immunol. 2008 Mar. 100(3 Suppl 3):S1-148. [Medline]. 

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